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Thursday, December 01, 2005
3:21 PM
the following passage is from a report on the prize-winning water strategy solution for miyako island developed by the teachers and students of miyako agriculture and forestry highschool. last week the swedish ambassador paid us a visit in recognition of last year`s first place win at the sweden world water forum: photos at http://www.flickr.com/photos/bombilladeluz/sets/1461967/
i am meant to be checking the english of the report. hmmm. chemistry class would have come in handy here. too bad i was never an ace at it-
2.2 Isolation, selection and mass culturing of phosphate-solubilization bacteria
In isolating phosphate-solubilization bacteria, we applied the soil dilution flat plate method and used soil composed of weathered Ryukyu limestone for the bacterial source. We placed 10g of the soil into 90 mL of distilled and autoclaved water contained in a 200mL Erlenmeyer flask, and mixed it for ten minutes. We inoculated 1 mL of the supernatant of the resulting mixture using a pipette into a sterilized plate containing hydroxyl apatite, and incubated it for ten days at the 28℃ in darkness11). We counted the colonies that formed a clear zone and converted the number into 1 g of dry soil12). We indicate phosphate-solubilization bacteria as bacteria that has the ability to solubilize phosphorus that had combined with positive iron and became non-soluble. Three roughly classified types such as hydrogen sulfide generating bacteria, sulfur oxidized bacteria, and organic acid generating bacteria are recognized12) 13).
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